The Functional Characterization of CCD Genes in Loquat
DOI:
https://doi.org/10.6911/WSRJ.202505_11(5).0002Keywords:
Loquat; CCD; expression pattern; function; zeaxanthin.Abstract
Carotenoid cleavage dioxygenase (CCD) is a pivotal enzyme that is widely distributed in plants, catalyzing the cleavage of carotenoids to produce a variety of apocarotenoid volatile compounds. However, the functional characterization of the CCD gene in loquat (Eriobotrya japonica) has not been thoroughly investigated. In this study, we compared 3-oxo-ionol glycosides in loquat and speculated that a highly expressed CCD enzyme in the leaves is responsible for carotenoid cleavage. We employed bioinformatics approaches to analyze the CCD gene family in loquat, constructing a phylogenetic tree and an expression heatmap. Based on subfamily classification and expression patterns, we inferred the potential functions of these genes. Three genes with high expression levels were identified, and their expression patterns correlated with the accumulation of carotenoid cleavage derivatives in loquat leaves. To validate their functions, we cloned these genes and successfully obtained two of them. And we confirmed that Eja10G000940.1 exhibits cleavage activity on zeaxanthin. This study lays a foundation for further analysis of the function of CCD genes in loquat.
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